Unveiling the Hidden Interactome of CRBN Molecular Glues with Chemoproteomics
- Developed a scalable affinity purification mass spectrometry workflow for the identification of chemically glued targets
- Screened 20 IMiD analogs and identified over 290 targets chemically recruited to CRBN. Targets were prioritized through computational alignment analysis
- The novel nonZF target PPIL4 was validated and the new selective lead molecule identified through a biochemical screen of over 6,000 IMiD analogs