HiBiT-SpyTag: A Minimal Tag for Covalent Protein Capture & Degrader Development

Time: 3:15 pm
day: Day 2 PM


  • We show a designed sized protein tag amenable to CRISPR/Cas9 knock-in which can be used for both protein quantification and covalent modification of a protein of interest
  • Using BRD4 as a proof-of-concept, we show efficient labelling of HiBiT-SpyTag labelled BRD4 with a SpyCatcher-dTAG protein, which can be degraded through various dTAG PROTACs
  • This tag was then used with IRE1α to develop the first PROTAC of this ER-membrane protein